Development of an Anti-Aging Poly Herbal Cream | Original Article

ABSTRACT:

The purpose of this research was to create a cream that has anti-aging properties by combining aqueous poly herbal extracts of four different plants Rosa damascena flowers (RDW), Clerodendrum paniculatum leaves (CPW), Murraya koengii leaves (MKW), and Annona squamosa leaves (ASW). Using an enzyme-linked immunosorbent assay (ELISA), researchers were able to verify that human dermal fibroblast (HDF) cells do contain collagen-I. CB 01, CB 02, and CB 03 are the three custom blends of plant extracts that were created. Collagen-I levels were significantly higher in the extract mix (CB03) at 18.65 0.061ngml (p 0.05) than they were at 16.53 0.064ngml for 100gml of normal ascorbic acid. Following the development of five distinct carrier cream bases (F-1, F-2, F-3, F-4, and F-5), the optimal formulation was found to be F2 (PHYAAC01) with (CB03) active blend (PHYAAC02), which demonstrated a collagen-I content of 17.53 0.011ngml in HDF cells. Using a neutral red uptake assay using NIH3T3 cell lines, the in vitro cytotoxicity of compounds (CB 03) and (PHYAAC02) was determined to be above 1000 gmL for a 24-hour study period. Physical and chemical parameters, such as pH and viscosity, were used to verify the final compositions' heat stability. Collagen-I gene expression was observed to be upregulated in the formulation (PHYAAC02) containing (CB 03) compared to the control and standard (market sample) formulations, suggesting that this ingredient may be useful for combating the ageing process and fostering the formation of a collagen matrix.