Article Details

Phytochemical Screening and Hypoglycemic Activity of Aqueous, Ethanolic and Methanolic Extracts of Phyllanthus Amarus and Catharanthus Roseus in Streptozotocin Induced Diabetic Rats | Original Article

Ramanna Krishna Murthy*, in Journal of Advances and Scholarly Researches in Allied Education | Multidisciplinary Academic Research

ABSTRACT:

The aim of the present study was to evaluate the hypoglycemic activity of Gymnema sylvestre and Phyllanthus amarus leaf extract in streptozotocin induced diabetic rats. Diabetes mellitus was induced by a single intraperitoneal injection of STZ (50 mgkg body weight). The experimental set up was formed as follows Group I–Normal Rats injected with normal saline is considered as vehicle control, Group II–Diabetic control administered with 20 Glucose Soln. for 14 days, Group III–STZ induced diabetic Wistar rats, administered with standard drug (Glibenclamide) 5 mgkg for 14 days, served as positive control, GROUPS-IV to IX STZ induced diabetic Wistar rats were orally administered with Aqueous, ethanolic and methanolic extracts of Phyllanthus amarus and Catharanthus roseus, at the dose of 300 mgkg rat body weight respectively. The results of the study indicates that Phyllanthus amarus and Catharanthus roseus extract significantly (P<0.01) reduced the blood sugar level. Though there are many approaches to control diabetes and its secondary complications, herbal formulations are preferred due to lesser side effects and low cost. Phyllanthus amarus and Catharanthus roseus are medicinal plants for the potential management of diabetes mellitus. The leaves are used in herbal medicinal preparation. The concerned study reveals the anti-diabetic potential of Phyllanthus amarus and Catharanthus roseus in controlling blood glucose level in Streptozotocin induced Diabetic rats. Bioactive compounds like Lighans and galloatnoids of Phyllanthus amarus and alkaloids such as (catharanthine, vindoline, vindolidine, vindolicine, vindolinine, vinblastine, vincristine, leurosine, and lochnerine) in Catharanthus roseus may be responsible for the significant stimulation of β-cells of pancreas in the production of Insulin. In the current work we have studied the anti-diabetic effect of the aqueous, ethanolic and methanolic extracts of Phyllanthus amarus and Catharanthus roseus on streptozotocin induced diabetic rats. The methanolic and aqueous extracts of Phyllanthus amarus, has significantly reduced the blood glucose levels than the ethanolic extract. Similarly, the ethanolic and methanolic extracts of Catharanthus roseus, has significantly reduced the blood glucose levels than the aqueous extract.