Article Details

Scanning, Characterising, Isolation and Testing Hypothesized Antimicrobial Peptide, (Wf24) From Chitin Binding Domain of Halorhabdus Tiamatea | Original Article

Talal Mabrouk Alanazi*, Saleh Mohammed Alamri, Abeer Abdulwahab Ahmed, Majed Ali Alafra, in Journal of Advances and Scholarly Researches in Allied Education | Multidisciplinary Academic Research

ABSTRACT:

Several studies have shown an increase in resistance to antibiotics. Hence, different approach in which the natural biological sources have been targeted to extract antimicrobial peptides AMPs. These AMPs should face less resistance by microbes and hence, are considered a novel approach in fighting these microbes. It has been reported by Bormann et al. 1999, that a similar chitin-binding domain has been isolated from Streptomyces Tendae, which possesses antifungal activity. The Purpose has been to scan and characterise the chitin-binding domain from Halorhabdus timatea and to isolate and test the candidate sequence derived from this domain. The sequence has been synthesised by NeoLab. Three servers, CAMP, APD Antibp2, which uses four logarithms, discriminant analysis (DA), Support Vector Machine (SVM), Artificial Neural Network (ANN) and Quantitative Matrices (QM), have been used to predict the antimicrobial activity of the sequence. Phyre2, Swiss-model and Loop servers have been used to predict the secondary structure of the candidate sequence. The programs used have predicted fairly similar results for the antimicrobial activity of the candidate sequence WF24. Structure prediction programs have predicted similar results, showing an alpha helix toward the N-terminus. The PPM server has shown a full immersion of the amphipathic alpha helix into the membrane. WF24 resides in the catalytic domain that belongs to glycoside hydrolase family 5. The naturally occurring end-tagging of the sequence WF24 with the hydrophobic residues tryptophan and phenylalanine, and the presence of glycine and (RW) motif within WF24, represent distinctive properties of a lot of AMPs in literature. Due to the limited time frame, WF24 has been tested against only 10 types of microbes, which decreases the chances of revealing antimicrobial activity. No positive results have been found. Because of the several advantages of WF24, further investigation using WF24, in its current form against several other types of microbes, should be performed.