Ergosteroland Cholesterol Supplementation Resulted In a Significant Increase (1-5- Fold)In the Sterol Content While Phospholipid Remained Unaffected In Microsporumgypseum. the Levels of Phosphatidylethanolamine and Phosphatidylcholineincreased In Ergosterol Supplemented Cells. However, a Decrease Inphosphatidylcholine and an Increase In Phosphatidylethanolamine Was Observed Incholesterol Grown Cells. the Ratio of Unsaturated to Saturated Fatty Acidsdecreased on Ergosterol/Cholesterol Supplementation. the Uptake of Amino Acids(Lysine, Glycine and Aspartic Acid) Decreased In Sterol Supplemented Cells.Studies With Fluorescent Probe L-Anilinonaphthalene-8-Sulfonate Showedstructural Changes In Membrane Organisation As Evident By Increased Number Ofbinding Sites In Such Cells..