Chromatographic Methods Utilize Partitioning a Sample Between Two Phases, One of Which Is the Stationary Phase and the Other One Is the Mobile Phase. Equipment For Liquid Chromatography Includes a Column, Where Separation Takes Places, Pump, Which Generates a Mobile Phase Flow, and Detector, Where the Separated Compounds Are Detected. Chromatography Is a Method Which Separates Molecules Based on Differences In Their Structures, Namely In Size, In Presence of Charged, Polar, and Non-Polar Groups or Moieties Interacting Specifically With an Affinity Column. After Separation In a Chromatographic Column, Particles Are Eluted to the Detector and They Are Detected As Chromatographic Peaks. There Are Several Physico-Chemical Principles of Separation Which Can Be Employed In Liquid Chromatography: Ion Exchange Chromatography, Which Separates Molecules According to Their Charges; Hydrophobic and Reversed Phase Chromatography, Which Separate According to Hydrophobicity of Molecules; Gel-Permeation Chromatography, Which Separates Molecules According to Their Size; and Affinity Chromatography, Which Is Based on Specific Interactions Between the Solute and Molecules Attached to the Column.