The Purpose of This Research Was to Create a Cream That Has Anti-Aging Properties By Combining
Aqueous Poly Herbal Extracts of Four Different Plants Rosa Damascena Flowers (Rdw), Clerodendrum
Paniculatum Leaves (Cpw), Murraya Koengii Leaves (Mkw), and Annona Squamosa Leaves (Asw). Using An
Enzyme-Linked Immunosorbent Assay (Elisa), Researchers Were Able to Verify That Human Dermal
Fibroblast (Hdf) Cells Do Contain Collagen-I. Cb 01, Cb 02, and Cb 03 Are the Three Custom Blends of Plant
Extracts That Were Created. Collagen-I Levels Were Significantly Higher In the Extract Mix (Cb03) at 18.65
0.061Ngml (P 0.05) Than They Were at 16.53 0.064Ngml For 100Gml of Normal Ascorbic Acid. Following The
Development of Five Distinct Carrier Cream Bases (F-1, F-2, F-3, F-4, and F-5), the Optimal Formulation Was
Found to Be F2 (Phyaac01) With (Cb03) Active Blend (Phyaac02), Which Demonstrated a Collagen-I
Content of 17.53 0.011Ngml In Hdf Cells. Using a Neutral Red Uptake Assay Using Nih3t3 Cell Lines, the In
Vitro Cytotoxicity of Compounds (Cb 03) and (Phyaac02) Was Determined to Be Above 1000 Gml For A
24-Hour Study Period. Physical and Chemical Parameters, Such As Ph and Viscosity, Were Used to Verify
The Final Compositions' Heat Stability. Collagen-I Gene Expression Was Observed to Be Upregulated In The
Formulation (Phyaac02) Containing (Cb 03) Compared to the Control and Standard (Market Sample)
Formulations, Suggesting That This Ingredient May Be Useful For Combating the Ageing Proces ...