According to Who One Third of the World Population Have Tuberculosis.The Present Study Was Undertaken to Compare the Efficacy of Fluroescent Stainwith Z-N Stain In the Diagnosis of Pulmonary. 793 Cases of Suspected Pulmonarytuberculosis Were Included In the Study. All Samples Were Screen For Acid Fastbacilli (Afb) By Z-N & Fluorescent Staining Methods. Positive Samplesdetected By Fluorescent Stain Were 121(15.22%) When Compared to Zn Stain71(8.95%). Conclusion Compared to Z-N Stain(8.95%). Flurochrome Staining Was Found to Be More Efficient (15.22%) In Afbdetection of Afb from Cases of Pulmonary Tuberculosis. Keywords: Acid-Fast Bacilli;Auramine-0; Fluorescence; Microscopy; Tuberculosis; Ziehl-Neelsen.