The Purpose of the Study Was to Investigate the Electrical Activity of Selected Muscles During Spike Using Electromyography on Inter-University Players of Volleyball. In Volleyball, Spike Is the Most Exciting Technique Which Every Player Likes. In This Study Fifteen Male Subjects Ranged from Eighteen to Twenty Four Years Were Selected As the Subjects from Lakshmibai National University of Physical Education, Gwalior. These All Players Had Represented Inter-University Level and They Had No Upper Extremity Injuries or any Bone or Joint Disparities Within the Past Years. By Reviewing the Literature and Consultation With Experts, the Research Scholar Carried Out an Intensive Study and Selected Variables Were Anterior Deltoid, Medial Deltoid, Biceps Brachii and Triceps Brachii. the Criterion Measure Adopted For This Study Was Measured By Bio Tech Thought Technology of Eight Channel. After a Brief Warm-Up, and Electrodes Placement Accoding to Seniam Guidelines and Then Participants Allowed to Perform the Spike. Three Times Data Were Recorded and the Best Was Considered For Data. Descriptive Statistical and Percentile Technique Were Used to Find Out the Muscles, Which Were More Significant During Contact Phase In Volleyball. the Level of Significance Was Set at 0.05. the Findings of the Study Clearly Revealed That Electrical Activity of Anterior Shoulder Muscle Showed Highest Activation Level of Emg During Contact Phase In Selected Muscle Groups. Anterior Shoulder Muscle and Tricep ...