A Study on Numerous Techniques of Chromatographic Evaluation | Original Article

ABSTRACT:

Chromatographic Evaluation Techniques utilize partitioning a sample between two phases, one of which is the stationary phase and the other one is the mobile phase. Equipment for liquid chromatography includes a column, where separation takes places, pump, which generates a mobile phase flow, and detector, where the separated compounds are detected. Chromatography is a method which separates molecules based on differences in their structures, namely in size, in presence of charged, polar, and non-polar groups or moieties interacting specifically with an affinity column. After separation in a chromatographic column, particles are eluted to the detector and they are detected as chromatographic peaks.