Study on Characterization of Plasmids from Bacteria that use Polycyclic Aromatic Hydrocarbons | Original Article

ABSTRACT:

Polycyclic aromatic hydrocarbons (PAH) are carcinogenic to humans, animals, and plants. Polycyclic aromatic hydrocarbon concentrations have been steadily growing in the environment, including water, soil, air, sediments, marine water, and vegetables. A sustainable method will be the formation of consortia of efficient polycyclic aromatic hydrocarbons degraders for the total removal of polycyclic aromatic hydrocarbons from polluted locations. The hydrocarbon polluted site soil sample and subsurface soil sample were utilised for bacterial species separation by enrichment.The 2,6-Dichlorophenol indophenol (2,6-DCPIP) test was used to screen for efficient polycyclic aromatic hydrocarbon degraders, and potential polycyclic aromatic hydrocarbon degraders were found using 16S-rDNA sequencing. The detected polycyclic aromatic hydrocarbons degrader was chosen for bioremediation research based on the catabolic route employed for catabolism of high molecular weight-polycyclic aromatic hydrocarbons such as pyrene and chrysene, as well as the examination of catabolic plasmid properties. On the basis of highest hydrocarbon degradability, consortia of high molecular weight-polycyclic aromatic hydrocarbons degrading bacteria were formed.The effective polycyclic aromatic hydrocarbon degraders were found to be Paracoccus denitrificans C5, Bacillus cereus C7, Rhodococcus pyridinivorans A1 and Pseudomonas stutzeri G11. Among the various bacterial consortia developed, the consortia generated from Paracoccus denitrificans C5 and Rhodococcus pyridinivorans C7 show the most promise. Hence it is concluded that the consortia formed by efficient polycyclic aromatic hydrocarbons digesting bacteria with new plasmids is effective for polycyclic aromatic hydrocarbon bioremediation.